Proteomic and metabolomic profiling reveal alterations in freezing-thawing and fresh drake sperm.

ABSTRACT: Cryopreservation of semen is crucial for preserving genetic resources and maintaining biodiversity. However, the unique structural characteristics of avian sperm make them particularly susceptible to cryoinjury, often resulting in impaired motility following thawing. Currently, research on drake semen cryopreservation remains limited. In this study, an integrated proteomic and untargeted metabolomic analysis was employed to investigate the molecular mechanisms underlying cryodamage-induced impairment of drake sperm motility. The findings revealed that glycolytic enzymes and peroxisomes play essential roles in maintaining sperm function during cryopreservation. Proteomic and metabolomic profiling of fresh (FS) and frozen-thawed (FT) semen samples from drakes identified a total of 6,946 prot

SUBMITTER: Wang S 

PROVIDER: S-EPMC12752543 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature