MnO₂ Nanosponge-Accelerated Cas12a Trans-Cleavage: Breaking the Kinetic Barrier for In Vivo RNA Imaging.

ABSTRACT: CRISPR/Cas12a system has emerged as a promising tool for in vitro biosensing, but its in vivo applications are hindered by its inefficient intracellular delivery and suboptimal trans-cleavage kinetics. To address these challenges, a Cas12a@MnO₂ nanosponge (hMNS) nanoprobe is constructed, in which hMNS as both a degradable carrier and an accelerator of CRISPR/Cas12a system for efficient imaging of RNA in living cells. The Cas12a@hMNS nanoprobe is obtained via a one-step co-assembly process. It not only facilitates synchronous cellular uptake and glutathione (GSH)-responsive release of CRISPR/Cas12a components, but also supplies adequate Mn²⁺ cofactors to improve the trans-cleavage activity of Cas12a. This dual-function probe can break the kinetic barrier of conventiona

SUBMITTER: Liu WJ 

PROVIDER: S-EPMC12752570 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature