Multimodal Imaging Reveals Cerebral Microcirculation Dynamics and Mechanisms in Mouse Central Nervous System After Intranasal Infection With Recombinant Pseudorabies Virus.

ABSTRACT: Neurotropic viruses are highly invasive in the central nervous system (CNS) and can disrupt the microcirculation, leading to neurological complications and encephalopathy. Understanding these pathophysiological alterations is crucial for uncovering the underlying mechanisms of viral infection, developing effective vaccines, and creating targeted therapies. However, studying the cerebral microcirculation in vivo during infection is challenging. This study engineered the pseudorabies virus (PRV) Fa-Luc using the EP0-sgRNA-hSPCas9 system to investigate cerebral microcirculation dynamics during PRV-induced CNS infection. Moreover, a multi-modality imaging strategy that integrates macroscale magnetic resonance imaging, bioluminescence with microscale laser speckles, optical coherence tomography

SUBMITTER: Ling S 

PROVIDER: S-EPMC12752574 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature