Compound Sophorae Decoction Alleviates Ferroptosis in Colitis Rats via Activating Keap1/Nrf2/GPX4 Signaling Pathway.

ABSTRACT:

Background

Compound sophorae decoction (CSD) has been extensively applied in clinic for the treatment of ulcerative colitis (UC). However, the effect and precise therapeutic mechanism have not been fully clarified. In this study, we systematically explored the protective efficacy and the underlying molecular mechanisms of CSD against UC.

Methods

The main constituents of CSD were analyzed by HPLC-MS/MS and TLC. H₂O₂-treated Caco-2 cells were employed to investigate the impact of CSD on ferroptosis and its underlying mechanism. The impacts of CSD on inflammation, oxidative stress, and ferroptosis in vitro were evaluated by ELISA, biochemical detection, or fluorescence probe. The UC model was established in rats by administering 5% DSS in the drinki

SUBMITTER: Wang J 

PROVIDER: S-EPMC12752875 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature