Sucrose but not arsenic induce hepatic steatosis which correlates with calpain-1 inhibition.

ABSTRACT: Exposure to arsenic or sugary drinks can increase the risk of developing metabolic dysfunction-associated steatotic liver disease (MASLD), although the mechanisms involved and its possible interactions remain unexplored. This work aimed to characterize the development of MASLD and their molecular mechanisms, induced in male Wistar rats consuming 20% sucrose (S), 50 ppm sodium arsenite (A), or both (A + S) through drinking water for 2 months. We found that rats consuming S developed liver steatosis and extensive fibrosis. Liver steatosis induced by S and A + S correlated with decreased calpain-1 protein abundance and calpain-dependent αII-spectrin proteolysis. We showed that calpain-1 inhibition in vitro induces lipid accumulation in hepatocytes probably by increasing PPARγ protein levels i

SUBMITTER: Velasco M 

PROVIDER: S-EPMC12752951 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature