Preparing Porcine Eyes for Confocal Reflectance Microscopy to Visualize the Vitreous Collagen Fiber Network.

ABSTRACT: The vitreous is a clear gel that fills the space between the lens and retina. Its structure is supported by a network of collagen fibers, which likely influences viscoelastic properties and contributes to retinal disease. However, the organization of this collagen network remains poorly characterized because imaging the vitreous in its native state is challenging. Many imaging techniques applied to the vitreous cause dehydration and distortion, and dye-based methods can introduce artifacts or nonspecific labeling, making it difficult to study the collagen network. In this protocol, we outline: 1) a method for dissecting porcine eyes for imaging; 2) a strategy for mounting the eyes on an inverted microscope; 3) guidelines for imaging the vitreous collagen network in its native state using c

SUBMITTER: Morgan DJ 

PROVIDER: S-EPMC12753011 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature