Femtosecond Laser Engraving Promotes the Repopulation of Decellularized Human Articular Cartilage.

ABSTRACT: Decellularized articular cartilage of human origin presents itself as the most homologous filling material for focal cartilage defects. Yet, the full repopulation of the exceptionally dense collagen construct has never been achieved without providing host cells with artificially created migration paths into the matrix. Within this study, we examine the use of a femtosecond laser to engrave fine patterns into human articular cartilage before decellularization and GAG depletion (decell-deGAG). Scaffolds were tested for decellularization success and mechanical behavior. Seeding tests were performed to assess biocompatibility and examine the performance in a simulated defect environment using an osteochondral plug model in vitro and in vivo in an ectopic nude mouse model. The composition and s

SUBMITTER: Schneider C 

PROVIDER: S-EPMC12753096 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature