Feasibility to infer accurate fibrinogen concentrations from thrombin generation, prothrombin time, or Reptilase time waveforms.

ABSTRACT:

Background

A rapid point-of-care test to measure fibrinogen levels is crucial to adequately treat major blood loss, eg, because of trauma, postpartum hemorrhage, or major surgery. Obtaining results from current fibrinogen assays is too time-consuming in emergency situations. We have developed mathematical methods to infer fibrinogen concentrations from the turbidity waveforms of the thrombin generation (TG), prothrombin time (PT), and Reptilase time (RT) assays.

Objectives

The aim of this study was to verify the performance of these methods using assay waveforms from real-world patient samples.

Methods

We measured TG, PT, and RT in 44 plasma samples with a wide range of fibrinogen levels (1.1-16.6 g/L), high D-dimer, prolonged PT, and/or containing anticoagulant medic

SUBMITTER: Arisz RA 

PROVIDER: S-EPMC12753218 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature