Supramolecularly gated enzymes: On-demand activity switching via host-guest competition for precision tumor therapy.

ABSTRACT: Dynamic control over enzyme function has been of great interest in the field of enzyme engineering and disease treatment, however, achieving on-demand regulation of native enzymes in living systems remains challenging due to the complexity of cellular environments and the lack of selective interactions. Here, we introduce a novel enzyme activity regulation system based on supramolecular host-guest recognition that enables reversible and on-demand regulation of native enzyme activity in living cells without covalent modification. This system utilizes the high-affinity interaction between cucurbit[7]uril (CB[7]) and amantadine (Ad) to precisely control enzyme function. CB[7] binds to key residues on the enzyme surface, blocking the active site and switching off enzyme activity. Upon intracel

SUBMITTER: Yang H 

PROVIDER: S-EPMC12753241 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature