ATF4 promotes renal tubulointerstitial fibrosis through hexokinase II-mediated glycolysis.

ABSTRACT:

Background

Renal tubulointerstitial fibrosis is a reliable predictor of progressive chronic kidney disease (CKD). Activating transcription factor 4 (ATF4) has recently emerged as a pivotal player in multiple pathophysiologic processes, particularly the stress response processes. This study aims to explore the role of ATF4 in tubulointerstitial fibrosis from a metabolic perspective.

Methods

A murine model of renal fibrosis was generated via unilateral ureteral obstruction (UUO). Quantitative PCR was employed to assess the expression of inflammation-related genes and fibrotic markers in renal tissue, while Western blotting was used to quantify the corresponding protein levels. Immunohistochemistry was performed to determine the localization and expression patterns of ATF4. Len

SUBMITTER: Feng S 

PROVIDER: S-EPMC12753322 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature