Advanced extraction techniques for sustainable recovery of health-promoting compounds from carob leaves.

ABSTRACT: Carob (Ceratonia siliqua) leaves (CSL) are an underutilized source of health-promoting compounds, including siliquapyranone, 1,2,3,6-tetragalloylglucose and myricitrin. CSL and its main compounds revealed remarkable antioxidant and hypoglycaemic potential. This study presents a systematic optimization of pressurized hot water extraction (PHWE) and probe-based ultrasound-assisted extraction (probe-UAE) as sustainable strategies for the recovery of CSL bioactive compounds. Using response surface methodology combined with UHPLC-UV profiling, the main parameters influencing extraction efficiency and compound stability were identified. Optimal PHWE (98 °C, three 5 min-cycles) and probe-UAE (ethanol 52 %, 100 g L^-1, 20 min) conditions ensured exhaustive recovery of target compounds wi

SUBMITTER: Serio S 

PROVIDER: S-EPMC12753491 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature