PTBP1 functions as a suppressor of ferroptosis in endometrial carcinoma cells by stabilizing SLC7A11 mRNA.

ABSTRACT: The RNA-binding protein polypyrimidine tract-binding protein 1 (PTBP1) facilitates tumor progression in various cancers by interacting with mRNAs of key oncogenes and tumor suppressors, thereby regulating their mRNA stability, alternative splicing, and protein translation. In this study, we found that​​ PTBP1 knockdown notably inhibited both in vitro cell viability and in vivo tumor growth in endometrial cancer (EC) cells. Elevated PTBP1 protein levels were also observed in endometrial cancer tissues compared to normal endometrial tissues. Transcriptomic sequencing revealed that PTBP1 depletion might impact lipid metabolism and ferroptosis in endometrial cancer cells. Through measurements of intracellular reactive oxygen species (ROS), glutathione (GSH), malondialdehyde (MDA), and Fe²

SUBMITTER: Zhu K 

PROVIDER: S-EPMC12753587 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature