Emergence of KPC-2 and NDM-13-coproducing carbapenem-resistant hypervirulent <i>Klebsiella pneumoniae</i> with high-risk sequence type ST11.

ABSTRACT:

Background

Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) co-producing KPC and NDM poses serious threats to public health. This study aimed to elucidate the molecular mechanisms underlying the resistance and virulence of a clinical CR-hvKP isolate coproducing KPC-2 and NDM-13 in China.

Methods

The K. pneumoniae strain A51998714 was isolated from a 58-year-old male patient in China in June 2024. MALDI-TOF/MS was used to species identity. Antimicrobial susceptibility testing was determined using the VITEK-2 system. Whole-genome sequencing (WGS), plasmid conjugation assays, and plasmid stability testing were conducted to characterize resistance and virulence determinants. Virulence potential was assessed using serum-killing assays.

Results</

SUBMITTER: Tao J 

PROVIDER: S-EPMC12753936 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature