Cryopreserved exosomes derived from hypoxic Wharton's jelly mesenchymal stromal cells enhance fibroblast proliferation, upregulate COL1A2 expression, and mitigate senescence.

ABSTRACT: Mesenchymal stromal cells (MSCs) and their exosomes support regeneration by modulating inflammation and promoting tissue repair. Hypoxic preconditioning enhances the regenerative potential of MSCs by enriching their secretome with trophic and immunomodulatory factors. This study evaluated the biological effects of cryopreserved exosomes from hypoxia-conditioned Wharton's Jelly MSCs (WJ-MSCs) on human dermal fibroblasts. WJ-MSCs were cultured under hypoxic conditions (5% O₂, 5% CO₂), and exosomes were isolated, characterized and cryopreserved. The initial preparation contained 1.5 × 10⁹ exosomes/mL (~350 μg/mL of total protein). Fibroblasts were treated for 72 h with three exosome dilutions 1:90 (~4 ng/μL), 1:30 (~12 ng/μL), 1:10 (~30 ng/μL), and their effects on proliferation (f

SUBMITTER: Quintero-Gil C 

PROVIDER: S-EPMC12753964 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature