FoCup, a secreted protein, is essential for virulence of <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> on cucumber.

ABSTRACT: Fusarium oxysporum f. sp. cucumerinum (Foc) infected cucumber (Cucumis sativus), leading to serious wilt disease and great economical losses worldwide. During infection, Foc secreted various protein effectors to facilitate colonization and disease development. Here, we identified a novel virulence effector, designated FoCup, which was highly up-regulated during Foc-cucumber interactions according to transcriptomic data. Bioinformatic analysis using SignalP-5.0 and InterPro predicted an N-terminal signal peptide and a cupredoxin domain in FoCup. Phylogenetic analysis indicated that FoCup is highly conserved within the Fusarium genus. Its secretory capability was experimentally confirmed by the yeast invertase secretion assay. Subcellular loca

SUBMITTER: Xu JT 

PROVIDER: S-EPMC12753967 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature