Secreted Glycoside Hydrolase BcGH61 From Botrytis cinerea Induces Cell Death by the Apoplastic Location and Triggers Intracellular Immune Perception.

ABSTRACT: Cell death-inducing proteins (CDIPs) are critical mediators of infection in the necrotrophic pathogen Botrytis cinerea, enabling host colonisation across its broad plant host range. Here, we identified a novel plant CDIP from B. cinerea, BcGH61, that exhibits dual activity: triggering extensive plant cell death while simultaneously promoting host defence responses. Functional analysis demonstrates that its full cell death-inducing capacity requires its apoplastic compartmentalisation, intact glycoside hydrolase activity, two conserved cysteine residues, and specific structural motifs spanning residues 193-240. Notably, this phytotoxic activity operates through a mechanism independent of the canonical immune coreceptors BAK1 and SOBIR1. During pathogenic invasion, bcgh61 demonstrates upregu

SUBMITTER: Zhu W 

PROVIDER: S-EPMC12754035 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature