Cathelicidin LL37-loaded extracellular vesicles from Edwardsiella piscicida promote antibacterial and wound-healing activity.

ABSTRACT: Bacterial extracellular vesicles (BEVs) have emerged as a promising therapeutic platform, offering the potential to be engineered for targeted molecular delivery. In this study, the human cathelicidin antimicrobial peptide (LL37) was encapsulated into extracellular vesicles derived from the fish pathogen Edwardsiella piscicida (EpEVs) through co-incubation. LL37-encapsulated EpEVs (EpEVs-LL37) were isolated by ultracentrifugation and characterized with a mean particle size of 73.6 ± 1.4 nm and a zeta potential of -11.27 ± 0.49 mV. Morphology of EpEVs-LL37 was confirmed as spherical-shaped particles. Enzymatic stability evaluation results revealed a proportionate increase in mean size and zeta potential with the pepsin concentration (0.4-2 mg/mL) at pH 2.0. EpEVs-LL37 exhibited lower toxici

SUBMITTER: Dias MKHM 

PROVIDER: S-EPMC12754054 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature