Enhancing delta-tocotrienol production in <i>Saccharomyces cerevisiae</i> via metabolic engineering strategies in conjunction with the mutagenesis of tocopherol cyclase.

ABSTRACT: Delta (δ)-tocotrienol is a member of the vitamin E family and exhibits bioactivities such as antioxidant, anti-inflammatory, and neuroprotective activities. As a nutrient with protective effects on human health, δ-tocotrienol has broad application prospects in food, cosmetic, and pharmaceutical industries. The construction of efficient microbial cell factories capable of δ-tocotrienol production using synthetic biology approaches is an effective strategy for supplementing or even replacing the vitamin E supply chain in the future. The current study successfully enhanced the biosynthesis of δ-tocotrienol in Saccharomyces cerevisiae by combining metabolic engineering and enzyme engineering strategies. Specifically, the substrate channel constructed by the sequential fusion of the enzy

SUBMITTER: Liu Z 

PROVIDER: S-EPMC12754398 | biostudies-literature | 2026 Jun

REPOSITORIES: biostudies-literature