Gut microbiota-derived tryptophan metabolite indole-3-carboxaldehyde enhances intestinal barrier function via aryl hydrocarbon receptor/AMP-activated protein kinase signaling activation.

ABSTRACT:

Objective

Intestinal inflammatory diseases significantly affect animal health, primarily by disrupting intestinal barrier function. Indole-3-carboxaldehyde (IAld), a key metabolite of tryptophan derived from gut microbiota, exhibits protective properties against intestinal inflammatory diseases. The regulatory mechanism by which IAld modulates intestinal barrier function requires further investigation.

Methods

An intestinal epithelial cell injury model was established by tumor necrosis factor-alpha (TNF-α) stimulation, alongside a mouse colitis model induced by dextran sulfate sodium (DSS) administration. Intestinal barrier function was assessed by immunoblotting, immunofluorescence, in vitro permeability assays, and histopathological analysis. Mitochondrial integrity and fu

SUBMITTER: Shi D 

PROVIDER: S-EPMC12754468 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature