Induction of germ cell-like cells from deleted in azoospermia-like enhanced green fluorescent protein gene knock-in chicken somatic cells via transgenic expression of pluripotency and germ cell-specific transcription factors.

ABSTRACT:

Objective

Germ cell identity is regulated by the coordinated action of multiple key transcription factors during embryonic development, which includes the induction and control of germ-line-specific gene expression. The expression of DEAD-box helicase 4 (DDX4) and deleted in azoospermia-like (DAZL) genes in chickens plays a pivotal role in germplasm formation and the specification of germ cell lineage from a totipotent genome. This study aimed to investigate the regulatory mechanisms underlying germ cell fate determination.

Methods

Large-scale gene expression profiling was conducted to screen and select critical transcription factors. This analysis identified differentially expressed genes in chicken primordial germ cells (PGCs), comprising 1,020 transcription factors. Addit

SUBMITTER: Lee BR 

PROVIDER: S-EPMC12754494 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature