Transcriptome and metabolite profiling reveals the mechanism of hepatic lipid metabolism during fasting in chicken.

ABSTRACT:

Objective

Since the liver is key to poultry lipid metabolism and fasting models are widely used in studying animal nutrition metabolism, this study used fasting to explore chicken liver lipid metabolism characteristics, providing a basis for poultry lipid metabolism research.

Methods

RNA-seq and metabolomics were combined to analyze 72-hour fasting effects on chicken liver lipid metabolism. Key lipid metabolism-related genes and metabolites were identified, with their mechanisms explored via RNAi and Oil Red O staining.

Results

Metabolomics identified 648 differential metabolites, including 8 (e.g., Arachidonoyl amine) with levels 160-fold higher than controls. Transcriptomics found 849 differentially expressed genes (DEGs), 78 involved in lipid metabolism; Protein-pr

SUBMITTER: Xu L 

PROVIDER: S-EPMC12754495 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature