<i>Cis</i>-2-decenoic acid modulates <i>Pseudomonas aeruginosa</i> virulence through a noncanonical transcriptional regulator.

ABSTRACT: Diffusible signal factor (DSF)-family quorum sensing (QS) signals are widely utilized by many pathogenic bacteria to modulate various biological functions and virulence. Previous studies showed that cis-2-decenoic acid (cis-DA) is involved in the modulation of biofilm dispersion in Pseudomonas aeruginosa, but the regulatory mechanism is unclear. Here, we report that cis-DA regulates the physiology and virulence of P. aeruginosa through FadD1, a long-chain fatty acid-CoA ligase. cis-DA specifically binds to FadD1 and enhances the binding ability of FadD1 to the target gene promoter DNA regions. Further analysis showed that FadD1 is a global regulatory factor that controls the transcription of various target genes. Moreover, FadD1 showed catalytic ac

SUBMITTER: Song S 

PROVIDER: S-EPMC12754631 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature