Whole-heart 3D reconstruction of mouse CVB3 myocarditis reveals spatial and transcriptomic heterogeneity of immune foci.

ABSTRACT:

Aims

Myocarditis is an inflammation of the myocardium and is characterized by poor prognosis in symptomatic patients and significant correlation with dilated cardiomyopathy (DCM). We investigated spatial, morphological, immunological, and transcriptomic heterogeneity of myocarditis inflammatory foci in the 3D space.

Methods and results

Utilizing a novel large tissue histological reconstruction workflow, CODA (not an acronym), we generated whole-heart 3D reconstructions of acute mouse Coxsackievirus B3 (CVB3) myocarditis at the single-cell resolution. Furthermore, we integrated immunohistochemical staining and spatial RNA-sequencing into the CODA workflow. This approach allowed the simultaneous morphological, immunological and transcriptional 3D analysis of acute mouse CVB3 m

SUBMITTER: Daoud A 

PROVIDER: S-EPMC12754843 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature