LncRNA MIR100HG induces degenerative changes in intervertebral disc degeneration nucleus pulposus cells by targeting miR-31-5p.

ABSTRACT:

Background

To investigate the regulatory role of LncRNA MIR100HG on the functional and inflammatory levels of nucleus pulposus cells (NPCs) in intervertebral disc degeneration (IDD).

Methods

A total of 183 patients (including 101 IDD patients) were included. Magnetic resonance imaging (MRI) assessed the degree of IDD. Intraoperative nucleus pulposus tissue was stored at - 80 °C. Logistic regression analysis identified risk factors for IDD. ROC analysis assessed the diagnostic value of LncRNA MIR100HG in predicting IDD. RT-qPCR detected gene expression, while CCK8 and flow cytometry observed cell proliferation and apoptosis. ELISA detected inflammatory factor expression. DLR and RIP validated the target relationship of genes.

Results

LncRNA MIR100HG is upregulated in N

SUBMITTER: Tian J 

PROVIDER: S-EPMC12754900 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature