TUG1: a potential endogenous reference gene for long noncoding RNA quantification in blood-based studies.

ABSTRACT: Long noncoding RNAs (lncRNAs) are promising biomarkers, but their accurate quantification by qPCR requires stable endogenous controls. In the present study, we aimed to identify suitable reference lncRNAs for normalization in whole-blood samples. We profiled the expression of 84 lncRNAs and eight commonly used mRNA reference genes by RT-qPCR in samples from 182 individuals. Transcript stability was assessed using three widely applied algorithms: geNorm, NormFinder and BestKeeper. Twenty-nine lncRNAs met predefined expression criteria: consistent detection across all samples, a maximum quantification cycle (Cq) below 33 and a median Cq below 30. Among these, TUG1 consistently ranked as the most stable candidate across all algorithms. FGD5-AS1 and ZFAS1 were also selected based on high stabi

SUBMITTER: Rodriguez-Munoz C 

PROVIDER: S-EPMC12754918 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature