Variant histone H2A.Z is globally localized to the promoters of inactive yeast genes and regulates nucleosome positioning.

ABSTRACT: H2A.Z is an evolutionary conserved histone variant involved in transcriptional regulation, antisilencing, silencing, and genome stability. The mechanism(s) by which H2A.Z regulates these various biological functions remains poorly defined, in part due to the lack of knowledge regarding its physical location along chromosomes and the bearing it has in regulating chromatin structure. Here we mapped H2A.Z across the yeast genome at an approximately 300-bp resolution, using chromatin immunoprecipitation combined with tiling microarrays. We have identified 4,862 small regions--typically one or two nucleosomes wide--decorated with H2A.Z. Those "Z loci" are predominantly found within specific nucleosomes in the promoter of inactive genes all across the genome. Furthermore, we have shown that H2A.

SUBMITTER: Guillemette B 

PROVIDER: S-EPMC1275524 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature