The Exoproteome and Surfaceome of Toxigenic <i>Corynebacterium diphtheriae</i> 1737 and Its Response to Iron Restriction and Growth on Human Hemoglobin.

ABSTRACT: Toxin-producing Corynebacterium diphtheriae strains are the etiological agents of the severe upper respiratory disease, diphtheria. A global phylogenetic analysis revealed that biotype gravis is particularly lethal as it produces diphtheria toxin and a range of other virulence factors, particularly when it encounters low levels of iron at sites of infection. To gain insight into how it colonizes its host, we have identified iron-dependent changes in the exoproteome and surfaceome of C. diphtheriae strain 1737 using a combination of whole-cell fractionation, intact cell surface proteolysis, and quantitative proteomics. In total, we identified 1414 of the predicted 2265 proteins (62%) encoded by its reference genome. For each protein, we quantified its degree of secretion and s

SUBMITTER: Goring AK 

PROVIDER: S-EPMC12755303 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature