Evidence for <italic>sult1st6y</italic> as a Major Candidate Sex-Determining Gene in Pacific Bluefin Tuna.

ABSTRACT:

Introduction

The Y chromosome-linked gene sult1st6y (a homolog of the estrogen sulfotransferase gene) has recently been identified in Thunnus, a genus characterized by an XX/XY sex determination system. This study examined whether sult1st6y is a sex-determining gene in bluefin tuna (Thunnus orientalis).

Methods

The expression of sult1st6y was examined using polymerase chain reaction analyses and in situ hybridization. Sult1st6y expression in masculinized XX testes produced by aromatase inhibitor administration and the expression profile of the sult1st6y mutant, which was produced using CRISPR/Cas9, were examined.

Results

Sult1st6y was specifically expressed in XY gonads during sex differentiation. The onset of sult1st6y expression preceded that of other genes promotin

SUBMITTER: Hayashida T 

PROVIDER: S-EPMC12755888 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature