High-resolution single-cell RNA sequencing using canFam4 reveals novel immune subsets and checkpoint programs in healthy dogs.

ABSTRACT:

Introduction

Single-cell RNA sequencing (scRNA-seq) enables high-resolution profiling of immune heterogeneity. Although previous studies have mapped the single-cell transcriptomic atlases of peripheral leukocytes in healthy dogs, the identification and functional characterization of distinct immune subsets remain incomplete.

Methods

We constructed a single-cell atlas of peripheral leukocytes from six healthy small-breed dogs using the 10x Genomics platform and the updated canFam4 genome.

Results and discussion

Analysis of 30,040 high-quality transcriptomes revealed 51 distinct immune subsets, including CD14 ⁺ CD33 ⁺ monocytes, XCR1 ⁺ CD1D ⁺ dendritic cells, CEACAM1 ⁺ CD24

SUBMITTER: Kim MC 

PROVIDER: S-EPMC12756145 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature