4-octyl itaconate modulates virulence-associated phenotypes and oxidative stress resistance in avian pathogenic Escherichia coli by targeting menB and wza.

ABSTRACT: Avian Pathogenic Escherichia coli (APEC) causes substantial economic losses to the poultry industry, primarily due to its diverse serotypes and extensive drug resistance. Itaconate, a metabolite of the tricarboxylic acid (TCA) cycle, demonstrates antimicrobial activity. However, the effect of itaconate on the biological characteristics of APEC remains unexplored. Because itaconate itself has poor membrane permeability, its cell-permeable derivative, 4-OI, has emerged as a promising alternative and has attracted significant attention. This study investigates the impact of 4-OI on the biological properties of APEC, including growth, biofilm formation, antioxidant stress response, and acid-base tolerance. The results revealed that the addition of 200 μM or 400 μM 4-OI to APEC94 cultures for 1

SUBMITTER: Che C 

PROVIDER: S-EPMC12756159 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature