A monoclonal antibody targeting B7-H3 in human embryonic stem cells exhibits anti-tumor activity in hepatocellular carcinoma xenografts.

ABSTRACT: B7-H3 is highly expressed with limited heterogeneity in various cancer types including hepatocellular carcinoma (HCC) but shows low expression in normal tissues. We generated monoclonal antibodies (MAbs) against surface molecules on human embryonic stem cells (hESCs) and found that NPB40, one of the MAbs, binds to HCC cells, but only weakly to primary hepatocytes. Immunoprecipitation with mass spectrometry identified that NPB40 recognizes B7-H3. In tumorsphere cultures, the expression of B7-H3 interacting with NPB40 was significantly increased, as were known cancer stem cell (CSC) markers. After cell sorting by NPB40, NPB40-high HCC cells showed higher clonogenic survival than NPB40-low HCC cells, suggesting B7-H3 as a potential CSC marker on HCC cells. NPB40 and Chi-NPB40, a chimeric form

SUBMITTER: Han SH 

PROVIDER: S-EPMC12756712 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature