Unveiling Differences in Bioavailability, Metabolism, and Excretion of Neohesperidin in Lean and Obese Rats Based on Liquid Chromatography-Tandem Mass Spectrometry.

ABSTRACT: Neohesperidin, the most abundant compound found in the flowers of Citrus aurantium L. var. amara Engl., has shown a significant therapeutic effect on obesity caused by a high sugar diet. Nevertheless, the pharmacokinetic differences of neohesperidin between lean and obese rats have not been evaluated, which is crucial information for its clinical application in treating obesity. In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established to explore the pharmacokinetic differences of oral administration (100 mg/kg) and intravenous injection (4 mg/kg) of neohesperidin in lean and obese rats. The absolute oral bioavailability of total neohesperidin in lean and obese rats was approximately 5.30% and 4.03%, respectively, with no significant statistical differ

SUBMITTER: Huang X 

PROVIDER: S-EPMC12756733 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature