A GH42 β‑Galactosidase from the Human Isolate <i>Bifidobacterium breve</i> DSM 20213: Biochemical and Transgalactosylation Properties Reveal the Potential for Galacto-oligosaccharides Synthesis.

ABSTRACT: A β-galactosidase (Bbreβgal-III) derived from the human isolate Bifidobacterium breve DSM 20213, which belongs to the glycoside hydrolase (GH) family 42, was successfully overproduced in Escherichia coli, purified to apparent homogeneity, and studied with respect to its biochemical and molecular properties as well as its transgalactosylation activity for the synthesis of galacto-oligosaccharides (GOS) in comparison to the previously reported two GH2 β-galactosidases, Bbreβgal-I and Bbreβgal-II, originating from this strain. Bbreβgal-III is a homotrimer with a molecular mass of approximately 240 kDa and shows distinct characteristics in terms of catalytic efficiency, pH dependence of activity and stability over a broad pH range, substrate specificit

SUBMITTER: Vu-Le KT 

PROVIDER: S-EPMC12756740 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature