The C2 Domain of PKC‑δ as a Dominant-Negative Modulator of Breast Cancer Cell Survival and Chemosensitivity.

ABSTRACT: Mounting evidence implicates Protein Kinase C-δ (PKC-δ) in breast cancer progression and therapy resistance. PKC-δ is activated by the second messenger diacylglycerol or by proteolytic cleavage, both of which expose the kinase's catalytic site and allow substrate phosphorylation. Furthermore, the C2 domain of PKC-δ regulates the kinase by mediating intra and intermolecular protein-protein interactions. Here, we investigated the autonomous effects of the PKC-δ C2 domain in two breast cancer cell lines, representing hormone-dependent and triple-negative breast cancer. A myc-tagged PKC-δ C2 domain (myc-δC2) was stably overexpressed in MCF-7 and MDA-MB-468 cells, and its effects on cell viability, apoptosis, and proliferation were assessed. myc-δC2 expression reduced cell viability and increas

SUBMITTER: Khader R 

PROVIDER: S-EPMC12756763 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature