Comparative N‑glycoproteomics Characterization of N‑glycoproteins of H3N8 Viruses Produced in Chicken Embryos versus MDCK Cells.

ABSTRACT: N-linked glycosylation has been demonstrated to play a critical role in the folding and immunomodulation of influenza virus glycoproteins. Chicken embryos have historically been the predominant production host for influenza vaccines; however, Madin-Darby canine kidney (MDCK) cells have recently emerged as a significant alternative host. N-glycosylation of H3N8 viruses produced in chicken embryos and MDCK cells was comparatively characterized by mass spectrometry-based N-glycoproteomics with isotopic labeling at the molecular level of intact N-glycopeptides. A total of 3647 and 912 site- and structure-specific intact N-glycopeptides with glycosite localization and N-glycan monosaccharide sequence structure confirmation were identified from the whole N-glycoproteome and hemagglutinin N-glyco

SUBMITTER: Wang H 

PROVIDER: S-EPMC12756775 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature