Imaging of Protein Assemblies up to 231 kDa in Tissues with Nano-DESI Mass Spectrometry.

ABSTRACT: Understanding the distribution of proteins and their assemblies in tissues is a major challenge in spatial biology. Mass spectrometry imaging (MSI) with nanospray-desorption electrospray ionization (nano-DESI) has previously enabled detection, imaging, and identification of intact protein complexes directly from tissues, including protein assemblies and pathological protein-metal complexes in neurodegenerative disease. To date, nano-DESI MSI has been most effective for lower molecular weight (MW) complexes (<100 kDa), with an upper limit of 113 kDa. Here, we demonstrate nano-DESI at molecular weights up to 231 kDa, more than doubling the previous limit, by combining nano-DESI with a new mass spectrometer system architecture designed for higher MW analysis. Both mouse brain and rat kidney t

SUBMITTER: Hale OJ 

PROVIDER: S-EPMC12756848 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature