Enhanced Sensitivity and Resolution in Biomolecular CEST NMR Experiments Using the Extended Hadamard Encoding Scheme.

ABSTRACT: By untangling information using deterministic frequency-domain linear combinations, the Hadamard Transform (HT) offers a robust way to extract spectral information. Hadamard-based encoding schemes can shorten the acquisition times of nuclear magnetic resonance (NMR) experiments, and lead to substantial sensitivity gains/unit time. However, the presence of spin-spin couplings, spin-spin cross-relaxation, or other mechanisms that break simple one-to-one relationships between a peak intensity and its frequency-domain position can complicate this approach. A case where manipulations at a given peak frequency position may affect the intensity of other peaks in the spectra arises in chemical exchange. Thus, in frequency-domain NMR experiments such as chemical exchange saturation transfer (CEST),

SUBMITTER: Kim J 

PROVIDER: S-EPMC12756851 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature