High-Wavenumber Raman Spectroscopy for the Identification and Biochemical Characterization of Microbial Species.

ABSTRACT: Raman spectroscopy is a powerful tool for microbiological and infectious disease research, enabling rapid discrimination of microbial species. While spectral discrimination has typically been performed using the fingerprint region (400-1800 cm^-1), high autofluorescence backgrounds can degrade signal quality and decrease the overall effectiveness. This work investigates the effectiveness of utilizing the low-background high-wavenumber region (2800-3800 cm^-1) to both identify and biochemically characterize microbial species. High-wavenumber spectra of 14 microbial species were collected and used to train and validate a multitiered classification model capable of identifying cell wall type (100%), genus (98.9%), and species (97.4%) with high accuracy. Additionally, utili

SUBMITTER: Walter AB 

PROVIDER: S-EPMC12756854 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature