Mitochondrial function and reactive oxygen species dynamics in Italian Mediterranean buffalo semen following cryopreservation and post-thaw incubation.

ABSTRACT:

Introduction

The current understanding of physiological parameters and redox balance in buffalo bull semen is limited and derived from various breeds. Moreover, the effects of cryopreservation in various buffalo breeds remain unclear.

Methods

This study aimed to investigate the relationships between physiological parameters and compare fresh (F) and frozen-thawed (T) semen in Italian Mediterranean buffalos (IMB; 7, bulls). Buffalo ejaculates were collected using an artificial vagina and cryopreserved using a standard protocol. Both F and T were analyzed by CASA and flow cytometry Semen parameters assessed included motility, viability (using PI or SG counterstains for each assay), acrosome integrity (PSA), mitochondrial membrane potential (JC1), mitochondrial integrity (MT),

SUBMITTER: Loddo G 

PROVIDER: S-EPMC12756934 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature