Genome-wide identification and characterization of the GDSL lipase gene family in <i>Dendrobium catenatum</i> and their potential role in drought stress tolerance and stomatal outer cuticular ledge formation.

ABSTRACT:

Background

Dendrobium catenatum, a drought-resistant medicinal orchid, exhibits unique adaptations to arid environments; however, the underlying molecular mechanisms remain largely unknow. The formation of the abnormal stomatal outer cuticular ledge (OCL) is prevalent in D. catenatum and is thought to contribute to its drought tolerance. Despite this, the GDSL lipases that regulate drought resistance in D. catenatum have not yet been identified. This study aimed to systematically identify the GDSL lipase family in D. catenatum, analyze their expression patterns, screen for candidates highly expressed in the leaf epidermis and stomatal guard cells, and validate their roles through drought tolerance assays and stomatal OCL characterization.

Methods

SUBMITTER: Tang J 

PROVIDER: S-EPMC12757301 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature