Nanopore-based amplicon sequencing for rapid detection and identification of <i>Bacillus</i> spp. in plant-based products.

ABSTRACT: Bacillus contamination in plant-based food products is a significant concern due to heat-resistant spores that can survive heating and proliferate during storage or handling, possibly leading to foodborne illnesses. Given the potential of high-throughput DNA sequencing to enhance microbial monitoring, we used a targeted approach by combining amplification of the Bacillus tuf gene with Oxford Nanopore sequencing. The ability of the MinION-based protocol to detect and identify closely related Bacillus species was first assessed using plant-based food samples spiked with spore suspensions of five representative Bacillus strains. A DNA extraction method, relying on food enzymatic pre-processing combined with mechanical cell lysis, was implemented to maximize Baci

SUBMITTER: Bisaschi M 

PROVIDER: S-EPMC12757441 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature