Double-layer NiCo heterostructure and enzyme-free dual-circle DNA signal amplification for simultaneous detection of dual target microRNAs of lung cancer using microfluidic electrochemical sensor.

ABSTRACT: Detection of microRNAs (miRNAs) reveals tumor information at the transcriptional level, which is crucial for early diagnosis and prognosis of tumors. However, the rapid detection of miRNAs in biological samples without relying on large equipment remains a significant challenge. In this work, a double-layer NiCo heterostructure (DL-H) for enhancing the reaction efficiency of DNA labels, and dual-target cycling reaction system were developed to improve detection sensitivity and shorten the detection time within 30 min. The DL-H and catechol provide favorable condition for methylene blue (MB) oxidation and single-electron transfer of ferrocene (Fc). The target cycling reaction allows one target to release multiple Fc/MB labels from the electrode surface, enhancing the signal differentiation t

SUBMITTER: Lai Q 

PROVIDER: S-EPMC12757455 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature