Quercetin mitigated LPS-induced PANoptosis in the bursa of Fabricius via the cGAS/STING signaling pathway in broilers.

ABSTRACT: Quercetin (QUE), a natural bioflavonoid with anti-inflammatory and antioxidant properties, regulates immune organ function by enhancing antioxidant capacity and maintaining mitochondrial homeostasis. This study aims to explore whether QUE can mitigate lipopolysaccharide (LPS)-induced PANoptosis in broilers bursal cells via cyclic GMP-AMP synthase (cGAS)/stimulator of interferon genes (STING) pathway. In vivo, White Leghorn chickens were assigned to Control, QUE (10 mg/kg gavage), LPS (0.15 mg/kg intraperitoneal injection), and LPS+QUE groups. In vitro, brolier lymphoma cells (DT40) were treated with LPS (40 μg/mL), QUE (10 μM), or their combination; STING agonist MSA-2 (2 μM) was used to verify pathway involvement. Hematoxylin-Eosin (H&E) staining, TUNEL assay, immunofluorescence, reactive

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12757475 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature