Gene regulatory network inference with popInfer reveals the dynamic regulation of hematopoietic stem cell quiescence.

ABSTRACT: Inference of gene regulatory networks (GRNs) can reveal cell state transitions from single-cell genomics data. However, obstacles to temporal inference from snapshot data are difficult to overcome. Single-nuclei multiomic data offer a means to bridge this gap and derive temporal information using joint measurements of gene expression and chromatin accessibility in the same single cells. We developed popInfer to infer networks that characterize lineage-specific dynamic cell state transitions from joint gene expression and chromatin accessibility data. Benchmarking against alternative methods for GRN inference, we showed that popInfer achieves higher accuracy in the GRNs inferred. popInfer was applied to study single-cell multiomics data characterizing hematopoietic stem cells (HSCs) and the

SUBMITTER: Rommelfanger MK 

PROVIDER: S-EPMC12757588 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature