CaMKKβ regulates transcription factor Elf2 gene methylation to maintain endothelial junctional barrier integrity.

ABSTRACT: The Ets transcription factors induce the expression of Tie2 and VE-cadherin. However, the regulation of these factors remains unknown. Here, we found that the gene encoding the Ets factor Elf2 was hypermethylated in Camkkβ-deficient (Camkkβ ^-/-) mice. Notably, these mice exhibited suppressed expression of Elf2, Tie2, and VE-cadherin. Additionally, either the depletion of CaMKKβ in human lung endothelial cells (EC) or EC-specific deletion of Camkkβ in mice led to a decrease in the expression of Elf2, Tie2, and VE-cadherin. In vivo inhibition of DNA methyltransferases or EC-specific expression of wild-type CaMKKβ restored the expression of Elf2, Tie2, and VE-cadherin in Camkkβ ^-/- mice. We find that methyl CpG binding protein MeCP2 repressed

SUBMITTER: Wang DM 

PROVIDER: S-EPMC12757589 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature