Initiator and elongator tRNA recognition mechanism in Mycobacterium tuberculosis methionyl-tRNA synthetase.

ABSTRACT: Protein synthesis is an essential target for anti-tubercular drug design. Methionyl-tRNA synthetase (MetRS) plays a crucial role in both the initiation and elongation phases of protein synthesis. Molecular recognition of the CAU anticodon of tRNA by MetRS during these two processes is a key step. To date, no experimental structures from Mycobacterium tuberculosis (Mtb) have revealed this binding. Therefore, we modeled the Mtb MetRS complexes with initiator and elongator tRNAs to find their differential binding mechanisms during molecular dynamics simulations of 9 microseconds. We found that the elongator tRNA formed a stable complex with the protein, whereas the initiator tRNA bound transiently. Nevertheless, major intra-tRNA interactions were maintained in both. This may reflect the rapid

SUBMITTER: Thakur S 

PROVIDER: S-EPMC12757634 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature