Design of B-Cell Multi-Epitope Subunit Vaccines Against <i>Glaesserella parasuis</i> by Reverse Vaccinology: An In Silico and In Vivo Study.

ABSTRACT: Glässer's disease caused by Glaesserella parasuis (GPS) is a severe disease that results in substantial economic losses to the swine industry worldwide. Here we describe a multiepitope vaccine cocktail (MEVC) that was designed using reverse vaccinology and immunoinformatics. The MEVC was comprised of three multiepitope subunits (MESs, designated as TB, 14B, and 24B), which were constructed using 14 B-cell epitopes predicted from six outer membrane antigens of GPS. The MESs exhibited non-allergenicity, high antigenicity, solubility, and stability. Predicted secondary and tertiary structures of the MESs were validated and showed strong binding affinity with the swine leukocyte antigen (SLA) by molecular docking. Immune simulation analysis further confirmed robust induction of both cel

SUBMITTER: Gong Y 

PROVIDER: S-EPMC12757848 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature