Differentiation between regulated and disrupted growth arrests allows tailoring of effective treatments for antibiotic persistence.

ABSTRACT: Antibiotic persistence, typically attributed to dormant bacteria, is known to be a major cause of treatment failure. However, despite many years of intense research, no clear consensus on its mechanism has emerged. Here, we demonstrate that high survival under antibiotics may originate from two fundamentally different growth-arrest archetypes: either from a regulated growth arrest, leading to a protected dormant cellular state, or from a dysregulated disrupted growth arrest. Using modeling and experimental approaches including transcriptomics, microcalorimetry, and microfluidics, we unveil the characteristics and vulnerabilities of each growth-arrest archetype. In particular, disrupted bacteria show a general impairment of membrane homeostasis. This understanding resolves previous conflict

SUBMITTER: Rotem A 

PROVIDER: S-EPMC12758538 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature