LTB4 Activates the MAP Kinase Pathway in Endothelial Cells to Cause Long-Lasting Neutrophil Tethering, MCP-1 and NO Releases.

ABSTRACT: Leukotriene B₄ (LTB₄), a potent chemotactic and immune-modulating eicosanoid, signals via two receptors (BLT₁ and BLT₂), leading to rapid but transient migratory, adhesive and secretory responses in phagocytes. Previously, we reported that BLT₁ is the predominating BLT in human umbilical vein endothelial cells (HUVEC). However, little is known about how ligation of these receptors affects adhesive and secretory endothelial responses over time. Here, we demonstrate that in HUVEC, LTB₄ dose-dependently and stereospecifically causes a biphasic tethering of neutrophils, where the second phase is robust, similar to that induced by lipopolysaccharide, and persists for 3-8 h. LTB₄ also causes up-regulation of E-selectin,

SUBMITTER: Johansson AS 

PROVIDER: S-EPMC12759030 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature