Intravital imaging of the formation and resolution of MHC class II-positive T-cell activation niches.

ABSTRACT: Intravital imaging has revealed many of the cellular interactions that regulate immune responses, but is limited by the number of cells that can be simultaneously identified and often restricted to analysis of a single time point. We have developed two new fluorescent reporter strains, IEbeta-mAmetrine and CD8beta-LSSmOrange, that faithfully label cells expressing MHC class II and CD8-positive conventional T cells, respectively. These fluorescent proteins are spectrally distinct from commonly used fluorescent proteins (GFP, YFP), and so, these mice can be used in combination with many previously created reporter mice. In addition, we established a protocol where we can sequentially image the same area of the ear dermis over several weeks without inducing additional inflammation. We applied

SUBMITTER: Oleksyn D 

PROVIDER: S-EPMC12759086 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature